abstract：:Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...

journal_title：Cell transplantation

authors： Boyce ST,Anderson BA,Rodriguez-Rilo HL

更新日期：2006-01-01 00:00:00

abstract：:Nonhuman primate models of Parkinson's disease (PD) have been invaluable to our understanding of the human disease and in the advancement of novel therapies for its treatment. In this review, we attempt to give a brief overview of the animal models of PD currently used, with a more comprehensive focus on the advantage...

journal_title：Cell transplantation

authors： Soderstrom K,O'Malley J,Steece-Collier K,Kordower JH

更新日期：2006-01-01 00:00:00

abstract：:End-stage organ failure is a major cause of death worldwide that can occur in patients of all ages and transplantation is the current standard of care for chronic end-stage disease of many organs. Despite the success of organ transplantation, it is becoming clear that there will never be enough organs made available t...

journal_title：Cell transplantation

authors： Triolo F,Gridelli B

更新日期：2006-01-01 00:00:00

abstract：:It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...

journal_title：Cell transplantation

authors： Dominguez-Bendala J,Pastori RL,Ricordi C,Inverardi L

更新日期：2006-01-01 00:00:00

abstract：:Parkinson's disease (PD), while primarily associated with degeneration of nigrostriatal dopamine neurons, is now increasingly recognized to have more widespread cell loss and so the most effective cell replacement therapy should target all these neuronal losses. Neural precursor cells might be ideal in this regard as ...

journal_title：Cell transplantation

authors： Jain M,Armstrong RJ,Elneil S,Barker RA

更新日期：2006-01-01 00:00:00

abstract：:The long-term durability of agarose microencapsulated islets against autoimmunity was evaluated in NOD mice. Islets were isolated from 6-8-week-old prediabetic male NOD mice and microencapsulated in 5% agarose hydrogel. Microencapsulated or nonencapsulated islets were transplanted into the omental pouch of spontaneous...

journal_title：Cell transplantation

authors： Kobayashi T,Aomatsu Y,Iwata H,Kin T,Kanehiro H,Hisanga M,Ko S,Nagao M,Harb G,Nakajima Y

更新日期：2006-01-01 00:00:00

abstract：:Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. Here we investigated an efficient method of hepatic differentiation from hES cells. A human ES cell line, KhES-1, was used and...

journal_title：Cell transplantation

authors： Chen Y,Soto-Gutierrez A,Navarro-Alvarez N,Rivas-Carrillo JD,Yamatsuji T,Shirakawa Y,Tanaka N,Basma H,Fox IJ,Kobayashi N

更新日期：2006-01-01 00:00:00

abstract：:Functional demands on a bioartificial liver support (BAL) device are not limited to biosynthetic activities, but must also encompass metabolic removal of potentially toxic substances. For most BALs, however, the concept and design are exclusively directed to biosynthetic support. To add the ability to metabolize and r...

journal_title：Cell transplantation

authors： Takahashi M,Sakurai M,Enosawa S,Omasa T,Tsuruoka S,Matsumura T

更新日期：2006-01-01 00:00:00

abstract：:The development of new cell replacement strategies using neural stem cells (NSC) may provide an alternative and unlimited cell source for clinical neural transplantation in neurodegenerative diseases such as Parkinson's and Huntington's disease. The clinical application of neural transplantation using NSC will therefo...

journal_title：Cell transplantation

authors： McLeod M,Hong M,Sen A,Sadi D,Ulalia R,Behie LA,Mendez I

更新日期：2006-01-01 00:00:00

abstract：:To engineer liver tissues with a clinically significant size, in vivo evaluation of performance using large-scale animal studies are necessary before proceeding to human clinical trials. As pigs are the most suitable candidates, the development of culture conditions suitable for porcine hepatocyte progenitors is very ...

journal_title：Cell transplantation

authors： Huang H,Hanada S,Kojima N,Sakai Y

更新日期：2006-01-01 00:00:00

abstract：:Human islet allografts are well known to induce full and sustained remission of hyperglycemia, with complete normalization of key metabolic parameters. Nevertheless, acquiring human islets, even from cadaveric human donor pancreases, remains a significant impediment to successful transplantation therapy for diabetes. ...

journal_title：Cell transplantation

authors： Luca G,Calvitti M,Nastruzzi C,Macchiarulo G,Becchetti E,Neri LM,Capitani S,Basta G,Brunetti P,Calafiore R,Cameron DF

更新日期：2006-01-01 00:00:00

abstract：:The mechanisms of donor hepatocyte integration into recipient liver are not fully understood. We investigated mechanisms of both the integration and interaction of transplanted hepatocytes with host liver cells as well as the repopulation of the host organ following intraportal transplantation. Mature hepatocytes were...

journal_title：Cell transplantation

authors： Koenig S,Stoesser C,Krause P,Becker H,Markus PM

更新日期：2005-01-01 00:00:00

abstract：:Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximi...

journal_title：Cell transplantation

authors： Yonekawa Y,Matsumoto S,Okitsu T,Arata T,Iwanaga Y,Noguchi H,Nagata H,O'Neil JJ,Tanaka K

更新日期：2005-01-01 00:00:00

abstract：:The aim of this study was to evaluate the qualitative change in reparative cartilage after autologous chondrocyte implantation (ACI). Ten knees of 10 patients were studied. The signal intensities of reparative and normal cartilage were evaluated by fat-suppressed three-dimensional spoiled-gradient recalled (FS 3D-SPGR...

journal_title：Cell transplantation

authors： Watanabe A,Wada Y,Obata T,Sasho T,Ueda T,Tamura M,Ikehira H,Moriya H

更新日期：2005-01-01 00:00:00

abstract：:Large-scale sterile methods for isolating hepatocytes are desirable for the development of bioartificial liver support systems. In this study the traditional centrifuge method was compared with the use of a Baylor Rapid Autologous Transfusion (BRAT) machine for isolating large quantities of porcine hepatocytes. After ...

journal_title：Cell transplantation

authors： Nieuwoudt MJ,Kreft E,Olivier B,Malfeld S,Vosloo J,Stegman F,Kunneke R,Van Wyk AJ,Van der Merwe SW

更新日期：2005-01-01 00:00:00

abstract：:Pancreatic islet transplantation is limited by shortage of donor organs. Although beta-cell lines could be used, their secretion of insulin is characteristically glucose independent and immunoisolation is required. Here we show that intrasplenic transplantation of encapsulated glucose-responsive mouse insulinoma cells...

journal_title：Cell transplantation

authors： Aoki T,Hui H,Umehara Y,LiCalzi S,Demetriou AA,Rozga J,Perfettit R

更新日期：2005-01-01 00:00:00

abstract：:Persistent corneal wounds result from numerous eye disorders, and to date, available treatments often fail to accelerate reepithelialization, the key initial step in wound healing. To speed reepithelialization, we explored a cell-transfer transplant method utilizing polydimethylsiloxane (PDMS) contact lenses to delive...

journal_title：Cell transplantation

authors： Pino CJ,Haselton FR,Chang MS

更新日期：2005-01-01 00:00:00

abstract：:Human marrow stromal cells (hMSCs) provide functional benefit in rats subjected to stroke. Astrocytes are coupled into a cellular network via gap junction channels, predominantly composed of connexin-43 (Cx43) proteins. Astrocytes are believed to play a vital role in neuroprotection by providing energy substrates to n...

journal_title：Cell transplantation

authors： Gao Q,Katakowski M,Chen X,Li Y,Chopp M

更新日期：2005-01-01 00:00:00

abstract：:Prior studies have suggested the possibility of immune-mediated death of xenogeneic hepatocytes in a bioartificial liver (BAL) during hemoperfusion. This study was designed to elucidate how immunity may cause death of xenogeneic hepatocytes in the BAL. Healthy dogs were treated with a BAL containing hollow fiber membr...

journal_title：Cell transplantation

authors： Nyberg SL,Amiot B,Hardin J,Baskin-Bey E,Platt JL

更新日期：2004-01-01 00:00:00

abstract：:Investigations indicate that an extract of green tea, polyphenol, can significantly increase the culture survival rate of rat islets without deteriorating their functionality. In this study, we examined the effect of adding polyphenol to islets isolated from human pancreata and nonhuman primate pancreata. Islets were ...

journal_title：Cell transplantation

authors： Zhang G,Matsumoto S,Hyon SH,Qualley SA,Upshaw L,Strong DM,Reems JA

更新日期：2004-01-01 00:00:00

abstract：:There are conflicting reports on the survival and immune reaction to allografts and xenografts of cultured skin substitutes (CSS). In this study, we investigated the allogeneic and xenogeneic responses to CSS of human keratinocytes and genetically engineered CSS expressing keratinocyte growth factor (KGF) that forms a...

journal_title：Cell transplantation

authors： Erdag G,Morgan JR

更新日期：2004-01-01 00:00:00

abstract：:Stem cell transplants into damaged myocardium may have the potential to improve cardiac function. We investigated the safety of transplanting unmanipulated autologous bone marrow into infarcted myocardium of patients undergoing coronary bypass surgery and assessed its efficacy to improve cardiac function. Fourteen pat...

journal_title：Cell transplantation

authors： Galiñanes M,Loubani M,Davies J,Chin D,Pasi J,Bell PR

更新日期：2004-01-01 00:00:00

abstract：:Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability stain...

journal_title：Cell transplantation

authors： Barnett MJ,McGhee-Wilson D,Shapiro AM,Lakey JR

更新日期：2004-01-01 00:00:00

abstract：:The present study was designed to evaluate the use of collagen gel loaded with human retinal pigment epithelium (ARPE19) in cellular transfer and to assess its viability within the gel. Collagen solution was prepared by dissolving calfskin in hydrochloric acid to make a final concentration of 2.0 mg/ml and this was mi...

journal_title：Cell transplantation

authors： Yeung CK,Chiang SW,Chan KP,Lam DS,Pang CP

更新日期：2004-01-01 00:00:00

abstract：:Previously, we found that hepatocyte growth factor receptor (c-Met)-and alpha-fetoprotein (AFP)-expressing cells were present in adult rat bone marrow, and that these cells also expressed hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. When bone marrow cells were cultured in a hepatocyte growth medium...

journal_title：Cell transplantation

authors： Miyazaki M,Masaka T,Akiyama I,Nakashima E,Sakaguchi M,Huh NH

更新日期：2004-01-01 00:00:00

abstract：:Hepatocyte transplantation has been proposed as a technique for bridging patients to whole-organ transplantation, for providing metabolic support during liver failure, and for replacing whole-organ transplantation in certain metabolic liver diseases. Assessment of hepatocyte engraftment has been difficult to measure, ...

journal_title：Cell transplantation

authors： Mas VR,Maluf DG,Thompson M,Ferreira-Gonzalez A,Fisher RA

更新日期：2004-01-01 00:00:00

abstract：:Adequate regulation of glucose levels by a microencapsulated pancreatic islet graft requires a minute-to-minute regulation of blood glucose. To design such a transplant, it is mandatory to have sufficient insight in factors influencing the kinetics of insulin secretion by encapsulated islets. The present study investi...

journal_title：Cell transplantation

authors： de Haan BJ,Faas MM,de Vos P

更新日期：2003-01-01 00:00:00

abstract：:After a unilateral striatal lesion, animals have generally been seen to have a bilateral impairment in paw reaching, with the contralateral paw being more affected. However, most studies to date have not used a pretraining paradigm to assess maximal capacity for paw reaching, to compare with any lesion-induced loss. T...

journal_title：Cell transplantation

authors： Fricker-Gates RA,Smith R,Muhith J,Dunnett SB

更新日期：2003-01-01 00:00:00

abstract：:Porcine neonatal islet-like cell clusters (NICCs) may be an attractive source of insulin-producing tissue for xenotransplantation in type I diabetic patients. We examined the functional and immunohistochemical outcome of the islet grafts in vitro during long-term culture and in vivo after transplantation to athymic nu...

journal_title：Cell transplantation

authors： Nielsen TB,Yderstraede KB,Schrøder HD,Holst JJ,Brusgaard K,Beck-Nielsen H

更新日期：2003-01-01 00:00:00

abstract：:Hepatocyte transplantation and artificial organ hepatic support require a number of functionally mature hepatocytes. However, their growth activity and functional behaviors are much smaller in culture after isolation from the liver. We examined whether continuously differentiating hepatocytes from multipotent hepatic ...

journal_title：Cell transplantation

authors： Suzuki A,Nakauchi H,Taniguchi H

更新日期：2003-01-01 00:00:00

abstract：:Several previous studies, suggesting the potential use of embryonic xenografts in the treatment of neurological disorders, indicate that neural growth and axonal guidance factors may function across species. In this light, blocks of fetal porcine neocortex were grafted into small cortical lesion cavities made in newbo...

journal_title：Cell transplantation

authors： Castro AJ,Meyer M,Møller-Dall A,Zimmer J

更新日期：2003-01-01 00:00:00

abstract：:Currently, different techniques to expand human hepatocytes in vitro are being investigated to generate enough cells for liver-directed cell therapies. However, based on observations in fibroblasts and other cell types, telomere attrition limits the proliferative capacity of normal somatic cells. Therefore, we explore...

journal_title：Cell transplantation

authors： Wege H,Chui MS,Le HT,Strom SC,Zern MA

更新日期：2003-01-01 00:00:00

abstract：:Liver cell transplantation into host organs like the spleen may possibly provide a temporary relief after extensive liver resection or severe liver disease or may enable treatment of an enzyme deficiency. With time, however, dedifferentiation or malignant transformation of the ectopically transplanted cells may be pos...

journal_title：Cell transplantation

authors： Lupp A,Danz M,Müller D

更新日期：2003-01-01 00:00:00

abstract：:Hepatocyte-based biological therapies are increasingly envisioned for temporary support in acute liver failure and provision of specific-liver functions in liver-based metabolic deficiency. One of the hurdles to develop such therapies is severe shortage of human livers for hepatocyte isolation. To address the issue, w...

journal_title：Cell transplantation

authors： Kobayashi N,Tanaka N

更新日期：2002-07-01 00:00:00

abstract：:Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure t...

journal_title：Cell transplantation

authors： Bicknese AR,Goodwin HS,Quinn CO,Henderson VCD,Chien SN,Wall DA

更新日期：2002-04-01 00:00:00

abstract：:A population of Hoechst 33342-stained cells, termed side population (SP) cells, can reconstitute the hematopoietic system of syngeneic mice. This study examined whether limiting numbers of SP cells can repopulate mice across a xenogeneic MHC class I barrier. SP cells were isolated from HLA.B7 and HLA.A2.1 transgenic m...

journal_title：Cell transplantation

authors： Jackson JD,Zhou G,Kuszynski CA,Cai J,Fox IJ

更新日期：2002-01-01 00:00:00

abstract：:The limited availability of human embryonic tissue for dopamine cell transplants in Parkinson's patients has led to an increased interest in using xenogeneic donor tissue. Unfortunately, without aggressive immunosuppression, such brain xenografts are rejected by the host immune system. Chronic brain xenograft rejectio...

journal_title：Cell transplantation

authors： Veng LM,Bjugstad KB,Freed CR,Marrack P,Clarkson ED,Bell KP,Hutt C,Zawada WM

更新日期：2002-01-01 00:00:00

abstract：:Fetal rat kidney cells produce high levels of glial-derived neurotrophic factor (GDNF) and exert neuroprotective effects when transplanted into the brain in animal models of Parkinson's disease and stroke. The purpose of the present experiment was to produce kidney cell lines that secrete GDNF. Genes encoding two trun...

journal_title：Cell transplantation

authors： Dillon-Carter O,Johnston RE,Borlongan CV,Truckenmiller ME,Coggiano M,Freed WJ

更新日期：2002-01-01 00:00:00

abstract：:The major limitation of nonhuman primate (NHP) embryonic stem (ES) cell research is inefficient genetic modification and limited knowledge of differentiation mechanisms. A genetically modified NHP-ES cell with biomarkers, such as green fluorescent protein (GFP), that allow noninvasive monitoring of transgenic cells, i...

journal_title：Cell transplantation

authors： Takada T,Suzuki Y,Kondo Y,Kadota N,Kobayashi K,Nito S,Kimura H,Torii R

更新日期：2002-01-01 00:00:00

abstract：:Although cells isolated from fetal liver are one of the major sources for liver tissue engineering, it is still very difficult to induce them to fully differentiate in vitro into mature hepatocytes. We therefore investigated the effects of nicotinamide (NA), dimethyl sulfoxide (DMSO), and oncostatin M (OSM) on differe...

journal_title：Cell transplantation

authors： Sakai Y,Jiang J,Kojima N,Kinoshita T,Miyajima A

更新日期：2002-01-01 00:00:00